Journal: BMC Cancer

Article Title: Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35

doi: 10.1186/s12885-025-14925-z

Figure Lengend Snippet: Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Article Snippet: The primary antibodies against USP35 (proteintech, Wuhan, China, 24559-1-AP), PKM2 (proteintech, 15822-1-AP), FPN (proteintech, 26601-1-AP), BIRC3 (proteintech, 24304-1-AP), β-actin (proteintech, 20536-1-AP) were incubated overnight at 4°C.

Techniques: Transfection

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: E3 ubiquitination ligase XIAP lightens diabetes‐induced cognitive impairment by inactivating TXNIP ‐ ERS ‐mediated neuronal injury

doi: 10.1002/kjm2.12913

Figure Lengend Snippet: E3 ubiquitination ligase XIAP was involved in the ubiquitination regulation of TXNIP protein. (A) The mRNA levels of BIRC3, CBLB, RBX1, and XIAP were measured in brain tissues from rats in the sham or DM group by qRT‐PCR. (B) The protein levels of BIRC3, CBLB, RBX1, and XIAP in the brain tissues from rats in the sham or DM group were measured by Western blotting. (C, D) The mRNA and protein levels of BIRC3, CBLB, RBX1, and XIAP in HG‐treated hippocampal neurons were analyzed by qRT‐PCR and Western blotting, respectively. (E) Co‐IP analysis confirmed the interaction between XIAP and TXNIP in hippocampal neurons. (F, G) qRT‐PCR detected the mRNA levels of XIAP and TXNIP in hippocampal neurons with lv‐XIAP or lv‐NC infection. (H) Western blotting examined the protein expressions of XIAP and TXNIP in hippocampal neurons with lv‐XIAP or lv‐NC infection. (I) Ubiquitination assays determined the TXNIP ubiquitination levels. Animal experiments were repeated five times, and cellular experiments were performed in triplicate. Results are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The primary antibodies including TXNIP (1:1000, #18243‐1‐AP), BIRC3 (1:1000, #24304‐1‐AP), CBLB (1:1000, #12781‐1‐AP), RBX1 (1:1000, #14895‐1‐AP), XIAP (1:1000, #10037‐1‐Ig), and GPR78 (1:1000, #11587‐1‐AP) were obtained from Proteintech.

Techniques: Ubiquitin Proteomics, Quantitative RT-PCR, Western Blot, Co-Immunoprecipitation Assay, Infection